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Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of APO-E inhuman liver tissue with APO-E antibody at 2.5 μg/ml.
Immunofluorescence of APO E in mouse brain tissue with APO E Antibody at 20 μg/mL. <br><br>Green: APO E antibody (7333) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Immunohistochemistry of APO E in mouse brain tissue with APO E Antibody at 5 μg/mL.
Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

APO-E Antibody

CATALOG NUMBER: 7333

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (78%)
Immunogen:
APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E.
The immunogen is located within amino acids 220 - 270 of APO-E.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 35 kDa
Observed: 34 kDa
Purification:
APO-E Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
APO-E Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
APO-E antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
APOE
Additional Names:
APO-E Antibody: AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E
Protein Accession Number:
NP_000032
Protein Gi Number:
4557325
Ncbi Gene Id Number:
348
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
APO-E Antibody: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Background Reference 1:
Vasquez EC, Peotta VA, Gava TMC, et al. Cardiac and vascular phenotypes in the epolipoprotein E-deficient mouse. J. Biomed. Sci. 2012; 19:22.
Background Reference 2:
Feussner G, Funke H, Weng W, et al. Severe type III hyperlipoproteinemia associated with unusual apolipoprotein E1 phenotype and epsilon 1/'null' genotype. Eur. J. Clin. Invest. 1992; 22:599-608.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7333

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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