Trial Size

ApoA1 Antibody

CATALOG NUMBER: 5097

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (83%), Bovine: (78%), Chicken: (71%)
Immunogen:
ApoA1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ApoA1.
The immunogen is located within the first 50 amino acids of ApoA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
ApoA1 antibody can be used for detection of ApoA1 by Western blot at 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Purification:
ApoA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
ApoA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ApoA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APOA1
Additional Names:
ApoA1 Antibody: Apolipoprotein A-I, Apolipoprotein A1, Apo-AI
Protein Accession Number:
P02647
Protein Gi Number:
113992
Ncbi Gene Id Number:
335
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ApoA1 Antibody: Apolipoprotein A1 (ApoA1) is the major protein component of high density lipoprotein (HDL) in plasma. ApoA1 is synthesized in the liver and small intestine and promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT), the enzyme responsible for the formation of most plasma cholesteryl esters. Defects in ApoA1 are associated with HDL deficiency, Tangier disease, and systemic non-neuropathic amyloidosis.
Background Reference 1:
Sorci-Thomas MG, Prack MM, Dashti N, et al. Differential effects of dietary fat on the tissue-specific expression of the apolipoprotein A-I gene: relationship to plasma concentration of high density lipoproteins. J. Lipid Res.1989; 30:1397-403.
Background Reference 2:
Lai C-Q, Parnell LD, and Ordovas JM. The APOA1/C3/A4/A5 gene cluster, lipid metabolism and cardiovascular disease risk. Curr. Opin. Lipid.2005; 16:153-66.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5097

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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