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Western blot analysis using Apoa5 monoclonal antibody against human serum total protein.
Western blot analysis using Apoa5 monoclonal antiobdy against truncated Apoa5 recombinant protein.
Western blot analysis using Apoa5 monoclonal antibody against human serum total protein.

Apoa5(ab) Antibody [1G5G9(c)]

CATALOG NUMBER: 32-109

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human APOA5 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot, ELISA.
Dilution:
Western Blot:1:2000 - 1:5,000
ELISA:Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Apoa5(ab) monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APOA5
Additional Names:
RAP3, UNQ411/PRO773, Apolipoprotein A5, Apo-AV, RAP3, APOAV
Protein Accession Number:
Q6Q788
Protein Gi Number:
60391728
Ncbi Gene Id Number:
116519
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50–70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-_ agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins.
Background Reference 1:
Pennacchio, L, Science 2001. 294, 169-173.
Background Reference 2:
Prieur, X. J Biol Chem 2003. 278, 25468-25480.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-109

List Size:
0.1 mg

List Price:

$515.00

Datasheet
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