APOBEC3D Antibody

CATALOG NUMBER: 43-122

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-ATDSQETRPGRA
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 38kDa band in lysates of cell lines A549 and HeLa after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.7kDa according to NP_689639.1. The 38kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APOBEC3D
Additional Names:
APOBEC3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D (putative), ARP6, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D
Protein Accession Number:
NP_689639.1
Protein Gi Number:
187608816
Ncbi Gene Id Number:
140564
Background Reference 1:
Stenglein MD, Harris RS.APOBEC3B and APOBEC3F inhibit L1 retrotransposition by a DNA deamination-independent mechanism.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-122

List Size:
0.1 mg

List Price:

$391.00

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