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46-731 (0.5ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-731 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-731 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-731 (0.5ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

APOBEC3G Antibody

CATALOG NUMBER: 46-731

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DEHSQDLSGRLR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 45kDa band observed in lysates of cell line Daudi (calculated MW of 46.4kDa according to NP_068594.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Kidney. Recommended concentration: 2.5ug/ml.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APOBEC3G
Additional Names:
APOBEC3G, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G, ARP9, CEM15, FLJ12740, MDS019, bK150C2.7, dJ494G10.1 , DNA dC->dU editing enzyme, OTTHUMP00000028911, apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G, phorbolin-like protein MDS019
Protein Accession Number:
NP_068594.1
Protein Gi Number:
13399304
Ncbi Gene Id Number:
60489
Background Reference 1:
Derse D, Hill SA, Princler G, Lloyd P, Heidecker G. Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc Natl Acad Sci USA. 2007 Feb 13;.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-731

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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