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43-462 (0.3ug/ml) staining of Human Kidney (A) and Liver (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-462 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-462 (0.3ug/ml) staining of Human Kidney (A) and Liver (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

apolipoprotein D Antibody

CATALOG NUMBER: 43-462

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ENGKIKVLNQE
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26kDa band observed in Human Liver and Kidney lysates (calculated MW of 21.3kDa according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.Enzyme immunoassay:This antibody is reported to work in sandwich-type ELISA both as capturer and as reporter antibody in combination with EB12281.
Predicted Molecular Weight:
Approx 26kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
apolipoprotein D
Additional Names:
APOD, apolipoprotein D, apo-D
Protein Accession Number:
NP_001638.1
Protein Gi Number:
114034
Ncbi Gene Id Number:
347
Background Reference 1:
Chen H, Li Y, Du J, Cao Y, Li X.Increased JNK1 activity contributes to the upregulation of ApoD in the apocrine secretory gland cells from axillary osmidrosis.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-462

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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