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45-269 (3.75ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-269 (3.75ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Apollon Antibody

CATALOG NUMBER: 45-269

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-PSSSKELPSDFQL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave no signal but low background in Human Liver and Kidney lysates at up to 1ug/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approx. 555KDa according to NP_057336.Immunohistochemistry:Paraffin embedded Human Lung. Recommended concentration: 3.75ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
BIRC6
Additional Names:
BIRC6, baculoviral IAP repeat-containing 6 (apollon), KIAA1289, baculoviral IAP repeat-containing 6, BRUCE, APOLLON, FLJ13726, FLJ13786, KIAA1289, ubiquitin-conjugating BIR-domain enzyme apollon
Protein Accession Number:
NP_057336.3
Protein Gi Number:
153792694
Ncbi Gene Id Number:
57448
Background Reference 1:
Chen Z, Naito M, Hori S, Mashima T, Yamori T, Tsuruo T. A human IAP-family gene, apollon, expressed in human brain cancer cells. Biochem Biophys Res Commun. 1999 Nov 2;264(3):847-54.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-269

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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