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42-971 (1ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-971 (1ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

APP Antibody

CATALOG NUMBER: 42-971

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: DAEFRHDSGYEVHHQK-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in Human Brain (Cerebral and Frontal cortex) lysates (calculated MW of 78.7kDa according to NP_958817.1 and 72.6 according to NP_001129601.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognize all reported isoforms of APP (NP_000475.1; NP_958816.1; NP_958817.1; NP_001129601.1; NP_001129602.1) and to recognize all lengths of the Amyloid beta peptide.
Positive Control 1:
Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Predicted Molecular Weight:
Approx 75 kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APP
Additional Names:
APP, amyloid beta (A4) precursor protein, AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, OTTHUMP00000096096, PN-II, alzheimer disease amyloid protein, amyloid beta A4 protein, beta-amyloid peptide, cerebral vascular amyloid peptide, peptidase nexin-II, preA4, protease nexin-II
Protein Accession Number:
NP_000475.1,NP_958816.1,NP_958817.1,NP_001129601.1,NP_001129602.1
Protein Gi Number:
4502167
Ncbi Gene Id Number:
351

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-971

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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