Appendix Lysate

CATALOG NUMBER: 1361

Tested Applications:
WB
Specifications
tissue-cell-type:
Appendix
Tissue Cell Type Expanded:
Human appendix tissue
Species:
Human
diagnosis:
Normal
Tested Applications:
WB
physical-state:
Liquid
Buffer:
Appendix lysate is supplied in SDS sample buffer containing 50 mM DTT.
Concentration:
2 mg/mL
Storage Conditions:
Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Background:
Appendix tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.

CATALOG NUMBER:

1361

List Size:
0.1 mg

List Price:

$146.00

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