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45-271 (0.5ug/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.
45-271 (0.5ug/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

APPL1 Antibody

CATALOG NUMBER: 45-271

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NRYSRLSKKRENDK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1.The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Rat Aortic Smooth Muscle Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
APPL1
Additional Names:
APPL1, DIP13alpha , adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
Protein Accession Number:
NP_036228.1
Protein Gi Number:
6912242
Ncbi Gene Id Number:
26060
Background Reference 1:
Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006 May;8(5):516-23. Epub 2006 Apr 16. Erratum in: Nat Cell Biol. 2006 Jun;8(6):642.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-271

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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