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45-272 (0.1ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-272 (3.8ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-272 (0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-272 (0.1ug/ml) staining of Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

APRIL Antibody

CATALOG NUMBER: 45-272

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KRKRETDDEGEDD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 28kDa band observed in Human Tonsil lysates, and approx 30kDa band observed in Rat and Pig Spleen lysates and in lysates of cell line NIH3T3 (calculated MW of 28.8kDa according to Human NP_006392.1, 31.1kDa according to Mouse NP_570959.1 and to Rat NP_571986.1 and 29.9kDa according to Pig XP_013849138.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining in lobular cells. Recommended concentration: 3-5ug/ml.
Specificity:
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ANP32B
Additional Names:
ANP32B, SSP29, APRIL, PHAPI2, acidic (leucine-rich) nuclear phosphoprotein 32 family, member B, acidic protein rich in leucines, OTTHUMP00000063770
Protein Accession Number:
NP_006392.1
Protein Gi Number:
5454088
Ncbi Gene Id Number:
10541
Background Reference 1:
Zhu L, Perlaky L, Henning D, Valdez BC. Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family. Biochem Mol Biol Int. 1997 Aug;42(5):927-35.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-272

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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