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Western blot analysis of extracts of various cell lines, using APTX antibody (19-618) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using APTX antibody (19-618).
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug APTX antibody (19-618). Western blot was performed from the immunoprecipitate using APTX antibody (19-618) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using APTX antibody (19-618) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

APTX Antibody

CATALOG NUMBER: 19-618

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 93-342 of human APTX (NP_001182178.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:20 - 1:50
Positive Control 1:
22Rv1
Positive Control 2:
A-549
Positive Control 3:
K-562
Positive Control 4:
BT-474
Positive Control 5:
U-87MG
Positive Control 6:
HT-29
Predicted Molecular Weight:
Observed: 40kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
APTX
Additional Names:
APTX, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT
Ncbi Gene Id Number:
54840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-618

List Size:
100 uL

List Price:

$436.00

Datasheet
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