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45-274 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-274 (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Arachidonate 5-lipoxygenase Antibody

CATALOG NUMBER: 45-274

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: C-ERNKKKQLPYYYLSPD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85kDa band observed in Human Bone Marrow and Rat Stomach lysates (calculated MW of 77.7.0kDa according to rat NP_036954). Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1475 - Rat Stomach Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ALOX5
Additional Names:
arachidonate 5-lipoxygenase, RP11-67C2.3, ALOX5, ALOX5, 5-LO, 5LPG, LOG5 , arachidonic acid 5-lipoxygenase, leukotriene A4 synthase,
Protein Accession Number:
NP_000689.1
Protein Gi Number:
4502057
Ncbi Gene Id Number:
240
Background Reference 1:
Kalayci O, Birben E, Sackesen C, Keskin O, Tahan F, Wechsler ME, Civelek E, Soyer OU, Adalioglu G, Tuncer A, Israel E, Lilly C. ALOX5 promoter genotype, asthma severity and LTC production by eosinophils. Allergy. 2006 Jan;61(1):97-103.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-274

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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