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Western blot analysis in Ramos cell line lysates (35ug/lane).
Western blot analysis of ARF5 using rabbit polyclonal ARF5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARF5 gene.
Western blot analysis in Ramos cell line lysates (35ug/lane).

ARF5 Antibody

CATALOG NUMBER: 58-942

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ARF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-108 amino acids from the Central region of human ARF5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARF5
Additional Names:
ADP-ribosylation factor 5, ARF5
Protein Accession Number:
P84085
Protein Gi Number:
51316990
Ncbi Gene Id Number:
381
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ADP-ribosylation factor 5 (ARF5) is a member of the humanARF gene family. These genes encode small guaninenucleotide-binding proteins that stimulate theADP-ribosyltransferase activity of cholera toxin and play a role invesicular trafficking and as activators of phospholipase D. Thegene products include 6 ARF proteins and 11 ARF-like proteins andconstitute 1 family of the RAS superfamily. The ARF proteins arecategorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 andARF5) and class III (ARF6). The members of each class share acommon gene organization. The ARF5 gene spans approximately 3.2kbof genomic DNA and contains six exons and five introns. [providedby RefSeq].
Background Reference 1:
Shiba, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(42):15416-15421(2006)
Background Reference 2:
Austin, C., et al. Biochemistry 41(14):4669-4677(2002)
Background Reference 3:
Shin, O.H., et al. Biochemistry 40(36):10846-10852(2001)
Background Reference 4:
Nevrivy, D.J., et al. J. Biol. Chem. 275(22):16827-16836(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-942

List Size:
400 ul

List Price:

$495.00

Datasheet
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