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Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μg/ml.
Immunohistochemistry of ARF6 in human kidney tissue with ARF6 antibody at 5 μg/mL.
Immunofluorescence of ARF6 in human kidney tissue with ARF6 antibody at 20 μg/mL.
Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 μg/ml.
Trial Size

ARF6 Antibody

CATALOG NUMBER: 7699

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen:
ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6.
The immunogen is located within the last 50 amino acids of ARF6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ARF6 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 19 kDa
Observed: 18 kDa
Purification:
ARF6 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ARF6 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ARF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ARF6
Additional Names:
ARF6 Antibody: ADP-ribosylation factor 6
Protein Accession Number:
NP_001654
Protein Gi Number:
4502211
Ncbi Gene Id Number:
382
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4).
Background Reference 1:
Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8.
Background Reference 2:
Moss J and Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999; 193:153-7.
Background Reference 3:
Cavenagh MM, Whitney JA, Carroll K, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-74.
Background Reference 4:
Schweitzer JK, Sedgewick AE, and D'Souza-Schorey C. ARF6-mediated endocytic recycling impacts cell movement, cell division and lipid homestasis. Semin. Cell Dev. Biol. 2011; 22:39-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7699

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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