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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody .HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

ARGFX Antibody

CATALOG NUMBER: 59-376

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ARGFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human ARGFX.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARGFX
Additional Names:
Arginine-fifty homeobox, ARGFX
Protein Accession Number:
A6NJG6
Ncbi Gene Id Number:
503582
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Homeobox genes encode DNA-binding proteins, many of whichare thought to be involved in early embryonic development. Homeoboxgenes encode a DNA-binding domain of 60 to 63 amino acids referredto as the homeodomain. This gene is a member of the ARGFX homeoboxgene family.
Background Reference 1:
Booth, H.A., et al. Gene 387 (1-2), 7-14 (2007) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-376

List Size:
400 ul

List Price:

$495.00

Datasheet
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