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46-626 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-626 (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-626 (0.3ug/ml) staining of A431 (A) and (1ug/ml) NIH3T3(B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-626 (0.01ug/ml) staining of Human Kidney (A) Mouse Liver (B) and (0.03ug/ml) Rat Kidney (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-626 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
46-626 (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Argininosuccinate Synthetase 1 Antibody

CATALOG NUMBER: 46-626

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ENPKNQAPPGLYTKTQD
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6kDa according to Mouse NP_031520.1 ). Recommended concentration: 0.03-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Specificity:
The variants represent identical protein (NP_000041.2 and NP_446464.1).
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ASS1
Additional Names:
Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase
Protein Accession Number:
NP_000041.2,NP_446464.1
Protein Gi Number:
53759107
Ncbi Gene Id Number:
445
Background Reference 1:
Hao G, Xie L, Gross SS. Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo. J Biol Chem. 2004 Aug 27;279(35):36192-200. Epub 2004 Jun 10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-626

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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