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Western blot analysis of ARHGAP39 in A20 cell lysate with ARHGAP39 antibody at (A) 1 and (B) 2 μg/mL
Western blot analysis of ARHGAP39 in A20 cell lysate with ARHGAP39 antibody at (A) 1 and (B) 2 μg/mL
Trial Size

ARHGAP39 Antibody

CATALOG NUMBER: 6445

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ARHGAP39 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ARHGAP39.
The immunogen is located within amino acids 880 - 930 of ARHGAP39.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ARHGAP39 antibody can be used for detection of ARHGAP39 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
ARHGAP39 antibody is predicted to not cross-react with other RhoGAP protein family members.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
ARHGAP39 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ARHGAP39 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ARHGAP39 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGAP39
Additional Names:
ARHGAP39 Antibody: CrGAP, Vilse, KIAA1688, Rho GTPase-activating protein 39
Protein Accession Number:
NP_079527
Protein Gi Number:
58331179
Ncbi Gene Id Number:
80728
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARHGAP39 Antibody: Rho GTPases are important regulators of the actin cytoskeleton and consequently influence the shape and migration of cells. GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins. ARHGAP39 (Rho GTPase activating protein 39) is a 1, 083 amino acid nuclear protein that contains one MyTH4 domain, one Rho GAP domain and two WW domains. It is encoded by a gene located on human chromosome 8, which is associated with a variety of diseases and malignancies. ARHGAP39 regulates robo signaling and plays important roles in axon guidance.
Background Reference 1:
Takai Y, Sasaki T, and Matozaki T. Small GTP-binding proteins. Physiol. Rev. 2001; 81:153-208.
Background Reference 2:
Hu H, Li M, Labrador JP, et al. Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. Proc. Natl. Acad. Sci. USA 2005; 102:4613-8
Background Reference 3:
Nusbaum C, Mikkelsen TS, Zody MC, et al. DNA sequence and analysis of human chromosome 8. Nature 2006; 439:331-5
Background Reference 4:
Lundström A, Gallio M, Englund C, et al. Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons. Genes Dev. 2004; 18:2161-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6445

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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