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Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (19-096) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody (19-096) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ARHGAP5 antibody (19-096) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody (19-096) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

ARHGAP5 Antibody

CATALOG NUMBER: 19-096

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1292-1501 of human ARHGAP5 (NP_001164.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
293T
Positive Control 4:
HepG2
Positive Control 5:
PC-12
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 185kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARHGAP5
Additional Names:
GFI2, RhoGAP5, p190-B, p190BRhoGAP, rho GTPase-activating protein 5, growth factor independent 2, p100 RasGAP-associated p105 protein, p105 RhoGAP, rho-type GTPase-activating protein 5
Ncbi Gene Id Number:
394
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-096

List Size:
100 uL

List Price:

$436.00

Datasheet
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