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Western blot analysis of ARHGDIA Antibody in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane)
Western blot analysis of ARHGDIA using rabbit polyclonal ARHGDIA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ARHGDIA Antibody in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane)

ARHGDIA Antibody

CATALOG NUMBER: 61-586

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This ARHGDIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-140 amino acids from the C-terminal region of human ARHGDIA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGDIA
Additional Names:
Rho GDP-dissociation inhibitor 1, Rho GDI 1, Rho-GDI alpha, ARHGDIA, GDIA1
Protein Accession Number:
P52565
Protein Gi Number:
1707892
Ncbi Gene Id Number:
396
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARHGDIA Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Background Reference 1:
Qiao,J., etc, Am. J. Physiol., Cell Physiol. 295 (5), C1161-C1168 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-586

List Size:
400 ul

List Price:

$495.00

Datasheet
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