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Western blot analysis in Jurkat cell line lysates (35ug/lane).
ARHGDIB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat cell line lysates (35ug/lane).

ARHGDIB Antibody

CATALOG NUMBER: 57-239

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ARHGDIB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-188 amino acids from the C-terminal region of human ARHGDIB.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGDIB
Additional Names:
Rho GDP-dissociation inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1
Protein Accession Number:
P52566
Ncbi Gene Id Number:
397
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the Rho (or ARH) protein family (see MIM165390) and other Ras-related small GTP-binding proteins (see MIM179520) are involved in diverse cellular events, including cellsignaling, proliferation, cytoskeletal organization, and secretion.The GTP-binding proteins are active only in the GTP-bound state. Atleast 3 classes of proteins tightly regulate cycling between theGTP-bound and GDP-bound states: GTPase-activating proteins (GAPs),guanine nucleotide-releasing factors (GRFs), and GDP-dissociationinhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rateof GDP dissociation from Ras-like GTPases (summary by Scherle etal., 1993 [PubMed 8356058]).
Background Reference 1:
Niu, H., et al. Oncol. Rep. 24(2):465-471(2010)
Background Reference 2:
Zhen, H., et al. Int. J. Gynecol. Cancer 20(3):316-322(2010)
Background Reference 3:
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Background Reference 4:
Said, N., et al. Cancer Metastasis Rev. 28 (3-4), 327-333 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-239

List Size:
400 ul

List Price:

$495.00

Datasheet
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