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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
ARHGEF3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

ARHGEF3 Antibody

CATALOG NUMBER: 62-060

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Monkey, Mouse
Immunogen:
This ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human ARHGEF3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGEF3
Additional Names:
Rho guanine nucleotide exchange factor 3, Exchange factor found in platelets and leukemic and neuronal tissues, XPLN, ARHGEF3
Protein Accession Number:
Q9NR81
Protein Gi Number:
52782760
Ncbi Gene Id Number:
50650
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rho-like GTPases are involved in a variety of cellularprocesses, and they are activated by binding GTP and inactivated byconversion of GTP to GDP by their intrinsic GTPase activity.Guanine nucleotide exchange factors (GEFs) accelerate the GTPaseactivity of Rho GTPases by catalyzing their release of bound GDP.This gene encodes a guanine nucleotide exchange factor, whichspecifically activates two members of the Rho GTPase family: RHOAand RHOB, both of which have a role in bone cell biology. It hasbeen identified that genetic variation in this gene plays a role inthe determination of bone mineral density (BMD), indicating theimplication of this gene in postmenopausal osteoporosis.Alternatively spliced transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Arthur, W.T., et al. J. Biol. Chem. 277(45):42964-42972(2002)
Background Reference 2:
Harrington, A.W., et al. J. Neurosci. 22(1):156-166(2002)
Background Reference 3:
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Background Reference 4:
Thiesen, S., et al. Biochem. Biophys. Res. Commun. 273(1):364-369(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-060

List Size:
400 ul

List Price:

$495.00

Datasheet
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