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45-281 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ARHGEF4 and probed with 45-281 (mock transfection in first lane).
45-281 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ARHGEF4 Antibody

CATALOG NUMBER: 45-281

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: PWEEPAGEKPSCS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARHGEF4
Additional Names:
ARHGEF4, Rho guanine nucleotide exchange factor (GEF) 4, ASEF, GEF4, STM6, KIAA1112, DKFZp434G2016, APC-stimulated guanine nucleotide exchange factor, ASEF1, Rho guanine nucleotide exchange factor 4
Protein Accession Number:
NP_056135.2,NP_127462.1
Protein Gi Number:
15011979
Ncbi Gene Id Number:
50649
Background Reference 1:
Thiesen S, Kubart S, Ropers HH, Nothwang HG. Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. Biochem Biophys Res Commun. 2000 Jun 24;273(1):364-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-281

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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