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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.

ARHGEF7 Antibody

CATALOG NUMBER: 61-358

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This ARHGEF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-688 amino acids from human ARHGEF7.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
90 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARHGEF7
Additional Names:
Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB
Protein Accession Number:
Q14155
Protein Gi Number:
50403776
Ncbi Gene Id Number:
8874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Background Reference 1:
Nagase T.,et al.DNA Res. 2:167-174(1995).
Background Reference 2:
Bagrodia S.,et al.J. Biol. Chem. 273:23633-23636(1998).
Background Reference 3:
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Background Reference 4:
Dunham A.,et al.Nature 428:522-528(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-358

List Size:
200 ul

List Price:

$495.00

Datasheet
Shipping Info

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