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Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (23-314) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using ARHGEF9 antibody (23-314) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using ARHGEF9 antibody (23-314). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ARHGEF9 antibody (23-314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ARHGEF9 Antibody

CATALOG NUMBER: 23-314

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-516 of human ARHGEF9 (NP_056000.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
22Rv1
Positive Control 3:
HeLa
Predicted Molecular Weight:
Observed: 61kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARHGEF9
Additional Names:
ARHGEF9, ARHDH9, EIEE8, HPEM-2, KIAA0424, PEM-2 homolog, PEM2, COLLYBISTIN, HPEM-2 collybistin, PEM-2
Ncbi Gene Id Number:
23229
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. Defects in this gene are a cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-314

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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