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Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.

Arhgef9 Antibody

CATALOG NUMBER: 61-443

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This Arhgef9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human Arhgef9.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Arhgef9
Additional Names:
Rho guanine nucleotide exchange factor 9, Collybistin, Rac/Cdc42 guanine nucleotide exchange factor 9, Arhgef9
Protein Accession Number:
Q9QX73
Ncbi Gene Id Number:
66013
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARHGEF9 belongs to a family of Rho-like GTPases that act as molecular switches by cycling from the active GTP-bound state to the inactive GDP-bound state. These proteins are key regulators of the actin cytoskeleton and are involved in cell signaling.
Background Reference 1:
Xiang,S., J. Mol. Biol. 359 (1), 35-46 (2006)
Background Reference 2:
Harvey,K., J. Neurosci. 24 (25), 5816-5826 (2004)
Background Reference 3:
Grosskreutz,Y., Biol. Chem. 382 (10), 1455-1462 (2001)
Background Reference 4:
Kins,S., Nat. Neurosci.(1), 22-29 (2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-443

List Size:
400 ul

List Price:

$495.00

Datasheet
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