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Western blot analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of ARID1A in mouse brain tissue with ARID1A antibody at 5 μg/mL.
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
Immunofluorescence of ARID1A in mouse brain tissue with ARID1A antibody at 20 μg/ml.
Western blot analysis of ARID1A in SK-N-SH cell lysate with ARID1A antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

ARID1A Antibody

CATALOG NUMBER: 8173

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.
The immunogen is located within amino acids 580 - 630 of ARID1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 133, 227, 251 kDa
Observed: 130, 260 kDa
Purification:
ARID1A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ARID1A
Additional Names:
AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1
Protein Accession Number:
NP_006006
Protein Gi Number:
21264565
Ncbi Gene Id Number:
8289
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
Background Reference 1:
Martens JA and Winston F. Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr. Opin. Genet. Dev. 2003; 13:136-42.
Background Reference 2:
Nie Z, Xue Y, Yang D, et al. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Mol. Cell. Biol. 2000; 20:8879-88.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8173

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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