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Western blot analysis of extracts of various cell lines, using ARID3A antibody (23-092) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ileum using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung cancer using ARID3A antibody (23-092) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 150ug extracts of MCF7 cells using 3ug ARID3A antibody (23-092). Western blot was performed from the immunoprecipitate using ARID3A antibody (23-092) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using ARID3A antibody (23-092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ARID3A Antibody

CATALOG NUMBER: 23-092

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 324-593 of human ARID3A (NP_005215.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Jurkat
Positive Control 2:
MCF7
Positive Control 3:
Mouse testis
Predicted Molecular Weight:
Observed: 95kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARID3A
Additional Names:
ARID3A, BRIGHT, DRIL1, DRIL3, E2FBP1
Ncbi Gene Id Number:
1820
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-092

List Size:
100 uL

List Price:

$436.00

Datasheet
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