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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

ARID4A Antibody

CATALOG NUMBER: 27-784

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARID4A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ARID4A antibody can be used for detection of ARID4A by ELISA at 1:62500. ARID4A antibody can be used for detection of ARID4A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
143 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ARID4A antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ARID4A
Additional Names:
ARID4A, RBP1, RBBP1, RBP-1, RBBP-1
Protein Accession Number:
NP_002883
Protein Gi Number:
115334677
Ncbi Gene Id Number:
5926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARID4A encodes a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described.
Background Reference 1:
Meehan, W.J., et al., (2004) J. Biol. Chem. 279 (2), 1562-1569.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-784

List Size:
100 ul

List Price:

$473.00

Datasheet
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