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Western blot analysis in CEM cell line lysates (35ug/lane).
ARL8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM cell line lysates (35ug/lane).

ARL8 Antibody

CATALOG NUMBER: 55-780

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ARL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-154 amino acids from the C-terminal region of human ARL8.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARL5B
Additional Names:
ADP-ribosylation factor-like protein 5B, ADP-ribosylation factor-like protein 8, ARL5B, ARL8
Protein Accession Number:
Q96KC2
Protein Gi Number:
34922029
Ncbi Gene Id Number:
221079
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARL5B (ARL8) belongs to a family of proteins that arestructurally similar to ADP-ribosylation factors (ARFs; see MIM103180). ARLs and ARFs are part of the RAS superfamily ofregulatory GTPases.
Background Reference 1:
Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)
Background Reference 2:
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Background Reference 3:
Sebald, E., et al. Gene 311, 147-151 (2003) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-780

List Size:
400 ul

List Price:

$495.00

Datasheet
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