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43-504 (2ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-504 (2ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ARNT Antibody

CATALOG NUMBER: 43-504

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: CNDKERFARSDDEQS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 90kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6kDa according to NP_001659.1). Recommended concentration: 2-4ug/ml.
Specificity:
This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
Predicted Molecular Weight:
Approx 90kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARNT
Additional Names:
ARNT, aryl hydrocarbon receptor nuclear translocator, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, OTTHUMP00000218217, OTTHUMP00000218218, OTTHUMP00000218220, class E basic helix-loop-helix protein 2, dioxin receptor, nuclear translocator, hypoxia-inducible factor 1, beta subunit
Protein Accession Number:
NP_001659.1,
Protein Gi Number:
114163
Ncbi Gene Id Number:
405
Background Reference 1:
Otsubo K, Kanegane H, Eguchi M, Eguchi-Ishimae M, Tamura K, Nomura K, Abe A, Ishii E, Miyawaki T.ETV6-ARNT fusion in a patient with childhood T lymphoblasticleukemia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-504

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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