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Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).hARNT immunoreactivity is localized to Nucleus significantly.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).hARNT immunoreactivity is localized to Nucleus significantly.

ARNT Antibody

CATALOG NUMBER: 57-640

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
87 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARNT
Additional Names:
Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2
Protein Accession Number:
P27540
Protein Gi Number:
114163
Ncbi Gene Id Number:
405
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The aryl hydrocarbon (Ah) receptor is involved in theinduction of several enzymes that participate in xenobioticmetabolism. The ligand-free, cytosolic form of the Ah receptor iscomplexed to heat shock protein 90. Binding of ligand, whichincludes dioxin and polycyclic aromatic hydrocarbons, results intranslocation of the ligand-binding subunit only to the nucleus.Induction of enzymes involved in xenobiotic metabolism occursthrough binding of the ligand-bound Ah receptor to xenobioticresponsive elements in the promoters of genes for these enzymes.This gene encodes a protein that forms a complex with theligand-bound Ah receptor, and is required for receptor function.The encoded protein has also been identified as the beta subunit ofa heterodimeric transcription factor, hypoxia-inducible factor 1. At(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusionprotein, is associated with acute myeloblastic leukemia.Alternative splicing results in multiple transcript variants.
Background Reference 1:
Otsubo, K., et al. Cancer Genet. Cytogenet. 202(1):22-26(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 4:
Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-640

List Size:
400 ul

List Price:

$495.00

Datasheet
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