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Western blot analysis in 293 cell line lysates (35ug/lane).
Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody . SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly.
Western blot analysis in 293 cell line lysates (35ug/lane).

ARNT2 Antibody

CATALOG NUMBER: 58-559

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ARNT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-278 amino acids from the Central region of human ARNT2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
79 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARNT2
Additional Names:
Aryl hydrocarbon receptor nuclear translocator 2, ARNT protein 2, Class E basic helix-loop-helix protein 1, bHLHe1, ARNT2, BHLHE1, KIAA0307
Protein Accession Number:
Q9HBZ2
Protein Gi Number:
125987793
Ncbi Gene Id Number:
9915
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of thebasic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily oftranscription factors. The encoded protein acts as a partner forseveral sensor proteins of the bHLH-PAS family, formingheterodimers with the sensor proteins that bind regulatory DNAsequences in genes responsive to developmental and environmentalstimuli. Under hypoxic conditions, the encoded protein complexeswith hypoxia-inducible factor 1alpha in the nucleus and thiscomplex binds to hypoxia-responsive elements in enhancers andpromoters of oxygen-responsive genes. A highly similar protein inmouse forms functional complexes with both aryl hydrocarbonreceptors and Single-minded proteins, suggesting addition roles forthe encoded protein in the metabolism of xenobiotic compounds andthe regulation of neurogenesis, respectively.
Background Reference 1:
Ramirez, J.M., et al. Eur. J. Immunol. 40(9):2450-2459(2010)
Background Reference 2:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 3:
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Background Reference 4:
Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-559

List Size:
400 ul

List Price:

$495.00

Datasheet
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