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45-289 (0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-289 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-289 (0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Aromatase Antibody

CATALOG NUMBER: 45-289

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HDLSLHPDETKN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in Human Placenta lysates (calculated MW of 57.9kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 4ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CYP19A1
Additional Names:
CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1 , ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, family 19, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase
Protein Accession Number:
NP_000094.2,NP_112503.1
Protein Gi Number:
13904858
Ncbi Gene Id Number:
1588
Background Reference 1:
Van Poznak CH, Hayes DF. Aromatase inhibitors for the treatment of breast cancer: is tamoxifen of historical interest only? J Natl Cancer Inst. 2006 Sep 20;98(18):1261-3. No abstract available.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-289

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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