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Western Blot at 1:1000 dilution + human uterus lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-CYP19A1 Pab in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human placenta tissue reacted with CYP19A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + human uterus lysate Lysates/proteins at 20 ug per lane.

Aromatase Antibody

CATALOG NUMBER: 63-003

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Aromatase (CYP19A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-485 amino acids from the C-terminal region of human Aromatase (CYP19A1).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CYP19A1
Additional Names:
Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19
Protein Accession Number:
P11511
Protein Gi Number:
117293
Ncbi Gene Id Number:
1588
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CYP19A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in the gene encoding CYP19A1 can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation.
Background Reference 1:
Xita,N.,Eur. J. Endocrinol. 158 (6), 861-865 (2008)
Background Reference 2:
Dos Santos,R.M.,(er) DNA Cell Biol. (2008) In press
Background Reference 3:
Nelson,D.R.,Pharmacogenetics 14 (1), 1-18 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-003

List Size:
400 ul

List Price:

$495.00

Datasheet
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