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45-291 staining (1ug/ml) of human heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-291 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-291 staining (1ug/ml) of human heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ARP1 Antibody

CATALOG NUMBER: 45-291

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-YEEDGSRAIHRKT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in human heart and brain lysates (predicted MW of 43kDa according to NP_005726). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5ug/ml.
Specificity:
This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ACTR1B
Additional Names:
ACTR1B, ARP1B, CTRN2, ARP1 actin-related protein 1 homolog B, centractin beta (yeast), PC3, centractin beta, ARP1, yeast homolog B, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1 actin-related protein 1 homolog B, centractin beta
Protein Accession Number:
NP_005726.1
Protein Gi Number:
11342680
Ncbi Gene Id Number:
10120
Background Reference 1:
Clark SW, Staub O, Clark IB, Holzbaur EL, Paschal BM, Vallee RB, Meyer DI. Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. Mol Biol Cell. 1994 Dec;5(12):1301-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-291

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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