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Western blot analysis of ARPC3 antibody in HL60 cell line lysates(35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARPC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of ARPC3 antibody in HL60 cell line lysates(35ug/lane)

ARPC3 Antibody

CATALOG NUMBER: 62-355

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ARPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-158 amino acids from the C-terminal region of human ARPC3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ARPC3
Additional Names:
Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC, ARPC3, ARC21
Protein Accession Number:
O15145
Ncbi Gene Id Number:
10094
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ARPC3 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein, the p21 subunit, has yet to be determined.
Background Reference 1:
Dubois,T., Nat. Cell Biol.(4), 353-364 (2005)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-355

List Size:
400 ul

List Price:

$495.00

Datasheet
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