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Western blot analysis of extracts of various cell lines, using ARSF antibody (23-400) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ARSF antibody (23-400) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ARSF antibody (23-400) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ARSF Antibody

CATALOG NUMBER: 23-400

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ARSF (NP_004033.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
A-549
Positive Control 3:
5637
Positive Control 4:
22Rv1
Positive Control 5:
BT-474
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 66kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARSF
Additional Names:
Arylsulfatase F, ASF, 316-, ARSF
Ncbi Gene Id Number:
416
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Multiple alternatively spliced variants, encoding the same protein, have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-400

List Size:
100 uL

List Price:

$419.00

Out of stock

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