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Western Blot at 1:4000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ART3 Antibody in HL60 cell line lysates (25ug/lane).
Western Blot at 1:4000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.

ART3 Antibody

CATALOG NUMBER: 61-368

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ART3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-310 amino acids from the C-terminal region of human ART3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ART3
Additional Names:
Ecto-ADP-ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, Mono(ADP-ribosyl)transferase 3, NAD(P)(+)--arginine ADP-ribosyltransferase 3, ART3, TMART
Protein Accession Number:
Q13508
Protein Gi Number:
22261808
Ncbi Gene Id Number:
419
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Background Reference 2:
Koch-Nolte, F., et al., Genomics 39(3):370-376 (1997).
Background Reference 3:
Levy, I., et al., FEBS Lett. 382(3):276-280 (1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-368

List Size:
400 ul

List Price:

$495.00

Datasheet
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