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42-639 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-639 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ARTN Antibody

CATALOG NUMBER: 42-639

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-NSTWRTVDRLS
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in lysates of cell lines HEK293, HepG2 and Kelly (calculated MW of 22.9kDa according to NP_003967.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pandey et al, Endocrinology. 2010 Mar;151(3):909-20; PMID: 20118197). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_003967.2; NP_476501.1; NP_476431.2). Reported variants represent identical protein: NP_476431.2, NP_001129687.1 Reported variants represent identical protein: NP_003967.2, NP_476432.2
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Positive Control 2:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3:
KELLY (Human neuroblastoma cell line) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARTN
Additional Names:
ARTN, artemin, ENOVIN, EVN, NBN, neublastin, neurotrophic factor, neurotrophic factor artemin
Protein Accession Number:
NP_003967.2,NP_476501.1,NP_476431.2
Protein Gi Number:
209954776
Ncbi Gene Id Number:
9048

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-639

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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