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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Thymus at 1.25 ug/ml.
Antibody used in IHC on Human kidney.

ASCL1 Antibody

CATALOG NUMBER: 27-561

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASCL1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ASCL1 antibody can be used for detection of ASCL1 by ELISA at 1:312500. ASCL1 antibody can be used for detection of ASCL1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ASCL1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ASCL1
Additional Names:
ASCL1, ASH1, HASH1, MASH1, bHLHa46
Protein Accession Number:
NP_004307
Protein Gi Number:
55743094
Ncbi Gene Id Number:
429
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.
Background Reference 1:
Somasundaram, K., et al., (2005) Oncogene. 24(47), 7073-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-561

List Size:
100 ul

List Price:

$473.00

Datasheet
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