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Western blot analysis of extracts of mouse pancreas, using ASF1A Antibody (22-288) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 20s.
Immunofluorescence analysis of HeLa cells using ASF1A antibody (22-288). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse pancreas, using ASF1A Antibody (22-288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.

ASF1A Antibody

CATALOG NUMBER: 22-288

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ASF1A (NP_054753.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
Mouse pancreas
Predicted Molecular Weight:
Observed: 23kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ASF1A
Additional Names:
ASF1A, ASF1 anti-silencing function 1 homolog A (S. cerevisiae), RP3-329L24.1, CGI-98, CIA, DKFZP547E2110, HSPC146, ASF1 anti-silencing function 1 homolog A, CCG1-interacting factor A, anti-silencing function 1A
Ncbi Gene Id Number:
25842
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-288

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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