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Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Ab-83).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using ASK1 (Ab-83).
Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).

ASK1 (Ab 83) Antibody

CATALOG NUMBER: 79-329

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ASK1 (Ab-83) antibody was raised against a peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total ASK1 protein.
Predicted Molecular Weight:
155 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP3K5
Additional Names:
ASK1, MEKK5, MAPKKK5, HER3, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5
Protein Accession Number:
NP_005914.1
Protein Gi Number:
5174547
Ncbi Gene Id Number:
4217
Background:
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Background Reference 1:
Mabuchi S, et al. (2004) Endocrinology. 145 (1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278 (26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21 (3): 893-901.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-329

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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