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Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.
Western blot analysis of ASMT Antibody in 293 cell line lysates (35ug/lane)
ASMT Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.

ASMT Antibody

CATALOG NUMBER: 61-665

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:500-1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASMT
Additional Names:
Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT
Protein Accession Number:
P46597
Protein Gi Number:
1170276
Ncbi Gene Id Number:
438
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.
Background Reference 1:
Melke, J., et al. Mol. Psychiatry 13(1):90-98(2008)
Background Reference 2:
Toma, C., et al. Mol. Psychiatry 12(11):977-979(2007)
Background Reference 3:
Ross, M.T., et al. Nature 434(7031):325-337(2005)
Background Reference 4:
Itoh, M.T., et al. Horm. Metab. Res. 35(3):153-157(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-665

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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