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Western blot analysis of extracts of various cell lines, using ASMTL antibody (22-637) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human gastric damage using ASMTL antibody (22-637) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug ASMTL antibody (22-637). Western blot was performed from the immunoprecipitate using ASMTL antibody (22-637) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using ASMTL antibody (22-637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ASMTL Antibody

CATALOG NUMBER: 22-637

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ASMTL (NP_004183.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
22Rv1
Positive Control 3:
SW620
Positive Control 4:
NIH/3T3
Positive Control 5:
HeLa
Positive Control 6:
Mouse pancreas
Predicted Molecular Weight:
Observed: 72kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ASMTL
Additional Names:
N-acetylserotonin O-methyltransferase-like protein, ASMTL, 211-, ASMTL
Ncbi Gene Id Number:
8623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene has an N-terminus that is similar to the multicopy associated filamentation (maf) protein of Bacillus subtilis and to orfE of Escherichia coli, while the C-terminus is similar to N-acetylserotonin O-methyltransferase. This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-637

List Size:
100 uL

List Price:

$436.00

Datasheet
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