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Antibody used in IHC on Human kidney.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in IHC on Human kidney.

ASPH Antibody

CATALOG NUMBER: 30-725

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASPH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ASPH antibody can be used for detection of ASPH by ELISA at 1:312500. ASPH antibody can be used for detection of ASPH by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ASPH antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ASPH
Additional Names:
ASPH, BAH, CASQ2BP1, HAAH, JCTN, junctin, AAH
Protein Accession Number:
NP_064549
Protein Gi Number:
9910364
Ncbi Gene Id Number:
444
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ASPH is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.This gene is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.
Background Reference 1:
Xian, Z.H., (2006) Mod. Pathol. 19 (2), 280-286.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-725

List Size:
100 ul

List Price:

$473.00

Datasheet
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