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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

ASS Antibody

CATALOG NUMBER: 29-557

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASS.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
ASS antibody can be used for detection of ASS by ELISA at 1:1562500. ASS antibody can be used for detection of ASS by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
45 kDa, 47 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ASS antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ASS1
Additional Names:
ASS, ASS1, CTLN1, DKFZp434P0216, ASS
Protein Accession Number:
NP_446464
Protein Gi Number:
16950633
Ncbi Gene Id Number:
445
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ASS catalyzes the penultimate step of the arginine biosynthetic pathway.The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Hao, G., (2004) J. Biol. Chem. 279 (35), 36192-36200.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-557

List Size:
100 ul

List Price:

$473.00

Datasheet
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