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Western blot analysis of ASS1 using rabbit polyclonal ASS1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASS1 gene.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
ASS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ASS1 using rabbit polyclonal ASS1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASS1 gene.

ASS1 Antibody

CATALOG NUMBER: 56-532

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This ASS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human ASS1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASS1
Additional Names:
Argininosuccinate synthase, Citrulline--aspartate ligase, ASS1, ASS
Protein Accession Number:
P00966
Protein Gi Number:
20141195
Ncbi Gene Id Number:
445
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene catalyzes the penultimatestep of the arginine biosynthetic pathway. There are approximately10 to 14 copies of this gene including the pseudogenes scatteredacross the human genome, among which the one located on chromosome9 appears to be the only functional gene for argininosuccinatesynthetase. Mutations in the chromosome 9 copy of ASS causecitrullinemia. Two transcript variants encoding the same proteinhave been found for this gene.
Background Reference 1:
Hozyasz, K.K., et al. Arch. Oral Biol. 55(11):861-866(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Kobayashi, E., et al. Mol. Cancer Ther. 9(3):535-544(2010)
Background Reference 4:
Tsai, W.B., et al. Mol. Cancer Ther. 8(12):3223-3233(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-532

List Size:
400 ul

List Price:

$495.00

Datasheet
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