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Western blot analysis of ATAD3B in human kidney tissue lysate with ATAD3B antibody at (A) 1 and (B) 2 μg/mL .
Immunohistochemistry of ATAD3B in human kidney tissue with ATAD3B antibody at 5 μg/mL.
Western blot analysis of ATAD3B in human kidney tissue lysate with ATAD3B antibody at (A) 1 and (B) 2 μg/mL .
Trial Size

ATAD3B Antibody

CATALOG NUMBER: 6389

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ATAD3B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ATAD3B.
The immunogen is located within amino acids 570 - 620 of ATAD3B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ATAD3B antibody can be used for detection of ATAD3B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
ATAD3B antibody is predicted to not cross-react with other ATAD protein family members. At least three isoforms of ATAD3B are known to exist.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
ATAD3B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ATAD3B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ATAD3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ATAD3B
Additional Names:
ATAD3B Antibody: TOB3, AAA-TOB3, KIAA1273, TOB3, ATPase family AAA domain-containing protein 3B
Protein Accession Number:
NP_114127
Protein Gi Number:
75677353
Ncbi Gene Id Number:
83858
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ATAD3B Antibody: ATAD3B is a member of the AAA ATPase family, a family of proteins that catalyze ATP into ADP and are involved in several cellular functions such as cell-cycle regulation, protein proteolysis and transport. The AAA ATPase family is characterized by a highly conserved AAA motif containing Walker homology sequences and imparting ATPase activity. Mitochondrial membrane proteins ATAD3A/B contribute to the stabilization of nucleoids and may participate in the transformation pathway and the chemosensitivity of oligodendrogliomas. The gene encoding ATAD3A/B/C maps to human chromosome 1 and has been suggested to be an anti-apoptotic marker.
Background Reference 1:
Patel S and Latterich M. The AAA team: related ATPases with diverse functions. Trends Cell Biol. 1998; 8:65-71.
Background Reference 2:
Ogura T and Wilkinson AJ. AAA+ superfamily ATPases: common structure—diverse function. Genes Cells 2001; 6:575-97.
Background Reference 3:
Hubstenberger A, Labourdette G, Baudier J, et al. ATAD3A and ATAD3B are distal 1p-located genes differentially expressed in human glioma cell lines and present in vitro anti-oncogenic and chemoresistant properties. Exp. Cell Res. 2008; 314:2870-83
Background Reference 4:
Fang HY, Chang CL, Hsu SH, et al. ATPase family AAA domain-containing 3A is a novel anti-apoptotic factor in lung adenocarcinoma cells. J. Cell Sci. 2010; 123:1171-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6389

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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