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Western blot analysis of extracts from various lysates, using Ataxin-3 antibody at 1:2000 dilution. | Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Western blot analysis of extracts from various lysates, using Ataxin-3 antibody at 1:2000 dilution. | Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

Ataxin-3 (ATXN3) Rabbit Recombinant Antibody (82-085)

CATALOG NUMBER: 82-085

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse brain,Rat brain
Predicted Molecular Weight:
41kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ATXN3
Additional Names:
AT3; JOS; MJD; ATX3; MJD1; SCA3; Ataxin-3 (ATXN3)
Protein Accession Number:
P54252
Ncbi Gene Id Number:
4287
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-085

List Size:
100 uL

List Price:

$477.00

Datasheet
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